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In <t>vivo</t> <t>osteochondral</t> regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed <t>3D</t> micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.
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In <t>vivo</t> <t>osteochondral</t> regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed <t>3D</t> micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.
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In <t>vivo</t> <t>osteochondral</t> regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed <t>3D</t> micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.
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In <t>vivo</t> <t>osteochondral</t> regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed <t>3D</t> micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.
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In vivo osteochondral regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed 3D micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.

Journal: Bioactive Materials

Article Title: Continuous mechanical-gradient hydrogel with on-demand distributed Mn 2+ /Mg-doped hydroxyapatite@Fe 3 O 4 for functional osteochondral regeneration

doi: 10.1016/j.bioactmat.2025.03.013

Figure Lengend Snippet: In vivo osteochondral regeneration capacity of gradient hydrogels. A) Gross images of the regenerated osteochondral tissues in the Control, UGel, HGGel, CGGgel, and CGGgel + MF groups, for 6 and 12 weeks, and B) ICRS macroscopic scores for gross observations. C) Surface geomorphologies of the regenerated osteochondral tissues at 12th week, and D) quantification of the representative roughness parameter Ra. E) Reconstructed 3D micro-CT images of the regenerated osteochondral tissues for 12 weeks. Analysis of micro-CT images showing F) the newly formed bone volume fraction (BV/TV), G) trabeculae number (Tb.N), and H) trabecular separation (Tb.Sp). I) Histological images of H&E and SO/FG staining of the regenerated osteochondral tissues for 6 and 12 weeks. J) Wakitani's scores and I) Seller's scores for histological evaluation. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 4.

Article Snippet: Biomimetic multilayer three-dimensional (3D) scaffolds are often designed to replicate the hierarchical structure of natural osteochondral tissue for the repair and regeneration of cartilage defects [ ].

Techniques: In Vivo, Control, Micro-CT, Staining